Multiplex RT-PCR Expression Analysis of Developmentally Important Genes in Individual Mouse Preimplantation Embryos and Blastomeres1

Expression profile of developmentally important genes in pre- and peri-implantation goat embryos produced in vitro

Background Little is understood about the regulation of gene expression during early goat embryo development. This study investigated the expression profile of 19 genes, known to be critical for early embryo development in mouse and human, at five different stages of goat in vitro embryo development (oocyte, 8-16 cell, morula, day-7 blastocyst, and day 14 blastocyst). MaterialsAndMethods Stage-...

Expression pattern of developmentally relevant genes in cattle preimplantation embryos

P-47: Effect of Blastocoelic Fluid Reduction on Quality and Expression of Developmentally Important Genes in Mouse Blastocysts

Background Recent researches reveal that manual puncturing of the trophectoderm of blastocyst before vitrification, increase the quality of embryo. However, in any of these studies, the importance of blastocoelic fluid and its impact on the formation of three cell lines is not mentioned. Therefore, in the present study, the effect of blastocoelic fluid reduction before vitrification on survival...

expression profile of developmentally important genes in pre- and peri-implantation goat embryos produced in vitro

background: little is understood about the regulation of gene expression during early goat embryo development. this study investigated the expression profile of 19 genes, known to be critical for early embryo development in mouse and human, at five different stages of goat in vitro embryo development (oocyte, 8-16 cell, morula, day-7 blastocyst, and day 14 blastocyst). materials and methods: st...

Optimization of real time RT-PCR methods for the analysis of gene expression in mouse eggs and preimplantation embryos.

This study was carried out to optimize conditions for using real time RT-PCR as an efficient and precise quantitative method for estimating the transcript levels of genes expressed in samples containing miniscule amounts of RNA, such as single mammalian oocytes and embryos. First, using mouse eggs and blastocysts, we tested three kinds of RNA isolation or collection methods: TRIZOL reagent, oli...